Sanger Sequencing Services in Hyderabad

What is Sanger Sequencing and Why It Remains the Gold Standard

Sanger sequencing, developed by Frederick Sanger in 1977 and also known as Chain‑termination or Dideoxy Sequencing, remains the benchmark for high‑precision DNA analysis. The approach uses labelled dideoxy nucleotides to terminate DNA synthesis at defined bases, generating fragment length patterns that directly reveal nucleotide order. Because each termination event corresponds to a specific base, Sanger sequencing delivers exceptionally accurate base calls commonly achieving up to 99.99% accuracy for single‑nucleotide variants and small indels.

Its targeted nature provides deep, unambiguous coverage of defined genomic regions, making it ideal for confirmatory testing, variant validation, and focused diagnostic assays where precision is critical. Although next‑generation sequencing platforms enable large‑scale, high‑throughput analyses, Sanger sequencing remains indispensable for clinical confirmation and small‑scale research due to its accuracy, interpretability, and broad acceptance by clinical laboratories, regulatory agencies, patent offices, and scientific journals.

Accuracy

0.99%

Accuracy for Point Mutations Detection

The highest precision available for confirming genetic variants and validating NGS results.

Scale

1 to ∞

Samples Per Project

Flexible throughput optimized for both single individual and large research cohorts.

Value

Rs 0

Per Reaction Cost

Cost-effective, highly accurate sequencing ideal for targeted analysis and specific regions.

Speed

10-0 Days

Typical Turnaround Time

Fast, reliable results delivered quickly to support timely clinical and research decision making.

Sanger and NGS: Complementary, Not Competitive

Feature	Sanger Sequencing	Next-Generation Sequencing
Accuracy	99.99%	95-99%
Target Scope	Specific genomic regions	Broad
Ideal Use	Validation, Confirmation	Diagnosis, Screening
Sample Size	Unlimited	100+ samples
Cost Per Sample	Rs 8,000	Rs 15,000 & above
Turnaround Time	10-15 working days	21-60 working days
Variant Confidence	Definitive gold-standard	High confidence
Clinical Confirmation	Reference standard	Requires Sanger validation

The Modern Approach: NGS finds variants across broad regions rapidly. Sanger confirms specific variants definitively. Both are complementary. Most cutting-edge genetic research uses both methods together. NGS screens, Sanger confirms.

Five Key Sanger Sequencing Applications

From validation to confirmation, Sanger serves diverse research and clinical needs.

NGS Validation

NGS identifies genetic variants. Sanger sequencing confirms them. Use Sanger to validate critical NGS findings before reporting to patients or publishing research.

Targeted Gene Screening

Screens specific disease genes cost-effectively. Perfect for hereditary disease screening when you know which gene variant to target.

Research Publication

Validate research findings with Sanger for publication. Peer reviewers expect Sanger validation of novel variants.

Haplotyping & Complex Variants

Determine which mutations are on the same DNA molecule. Clarify inheritance patterns and clinical severity through haplotype analysis.

Regulatory & Patents

Regulatory submissions and patent applications require gold-standard Sanger validation. Provide definitive genetic evidence for approval.

How Sanger Sequencing Works

A Five-step process from DNA to Definitive Sequence

PCR Amplification

The target genomic region is amplified using Polymerase Chain Reaction (PCR), generating millions of copies to provide sufficient DNA for sequencing.

DNA Denaturation

The amplified DNA is denatured into single strands and combined with fluorescently labeled dideoxynucleotides (ddNTPs), which terminate DNA synthesis at specific bases during the sequencing reaction.

Enzymatic Sequencing

DNA polymerase synthesizes new DNA strands. Incorporation of a ddNTP terminates strand extension, producing a collection of DNA fragments of varying lengths.

Capillary Electrophoresis

The DNA fragments are separated by size using capillary electrophoresis. Fluorescent signals from the labeled bases are detected and converted into a chromatogram for sequence analysis.

Sequencing Analysis & Reporting

Chromatogram data analyzed. DNA sequence determined base-by-base. Variants identified. Comprehensive report provided.

Why Researchers & Clinicians Choose Progenics for Sanger Sequencing

Excellence, experience, and reliability in every reaction

Progenics Difference

Uncompromised Reliability

We deliver industry-leading precision and strict quality controls. Our experienced scientists ensure every reaction meets the highest standards for absolute clarity in your clinical and research projects.

100%

Reliable Results

Technical Excellence

Latest Sanger instruments with highest fidelity. Automated fluorescent sequencing. Quality control exceeding industry standards.

10,000+ Reactions

Extensive sequencing experience with consistent quality and reliable results. Supporting research through 50+ scientific publications and trusted by leading academic and research institutions.

Rapid Turnaround Time

Standard turnaround time of 10-15 working days from sample receipt. Expedited 3-5 day processing available. Flexible batch testing optimized for your timeline.

Comprehensive Reporting

Detailed sequence reports with high-quality chromatograms. Clinical interpretation when applicable. Publication-ready results.

Expert Support

Sanger specialists available to discuss projects. Technical consultation. Project planning and optimization support.

Quality Assurance

Positive and negative controls on every run. Rigorous quality metrics tracked for every reaction.

Impact Stories from Researchers & Clinicians

"Sanger confirmation of our NGS findings was crucial for publication. Nature Neuroscience peer reviewers were confident in our variant calls because of Sanger validation. Progenics Sanger was essential to our success."

Research Team, Autism Genetics Study

High-impact journal publication enabled by Sanger validation

"We identified a potential disease-causing variant in our patient via exome sequencing. Before giving genetic diagnosis to the family, Sanger confirmed the variant. The confirmation gave us confidence in the diagnosis."

Clinical Genetics Laboratory

Clinical confidence and patient diagnosis enabled by Sanger

"Patent examiner requested validation of our diagnostic assay. Sanger Sequencing data from Progenics provided the gold-standard evidence needed. Patent was granted based on Sanger validation."

Diagnostic Company Founder

Patent approval and commercial product enabled by Sanger

Frequently Asked Questions About Sanger Sequencing

99.99% accuracy for point mutations and small insertions/deletions in sequenced regions. This exceptional accuracy makes Sanger the reference standard. No method is more accurate for small variants in specific regions.

Complementary methods. NGS screens broad regions rapidly. Sanger confirms specific variants with definitive accuracy. Both are essential. NGS finds variants, Sanger confirms them. Most modern genetic research uses both.

Efficiently detects point mutations (single base changes) and small insertions/deletions (up to few bases). Larger variants possible with special methodology. Contact team to discuss your specific variant size.

Standard turnaround time is 10-15 working days from sample receipt. Expedited 3-5 day service available for urgent projects. Batch projects can be optimized for timeline. Contact team for specific scheduling.

Rs 8,000 per reaction depending on sample count, genomic region size, and analysis complexity. Batch discounts available. Contact for detailed quote tailored to your project.

Absolutely. Sanger is the gold standard for publication validation. Nature and top journals expect Sanger confirmation of NGS findings. Progenics Sanger results are publication-ready.

Contact Progenics to discuss your project. Submit genomic DNA samples in recommended vials. Provide relevant information about target region or mutation. Team provides specific submission instructions.

Genomic DNA samples preferred (50-200 ng/µL, A260/A280 ratio 1.8-2.0). Minimum 500 ng per target region. Alternative samples accepted (cultured cells, tissue, blood, saliva). Discuss specific requirements with team.

Sanger Sequencing: The Gold Standard for DNA Sequencing